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KMID : 0525720150200040260
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Journal of Chitin and Chitosan
2015 Volume.20 No. 4 p.260 ~ p.266
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The Effect of the Extracted Celastrus orbiculatus Fruits on the Water-soluble Chitosan`s Antioxidant Activitiy
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Kim So-Hee
Jung Byung-Ok
Chung Suk-Jin
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Abstract
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This study has implemented to study whether an antioxidant activity appears when water-soluble chitosans(WSCs) react with the extract of Celastrus orbiculatus fruits. A total polyphenol experiment was conducted to analyze phenolic contents of Celastrus orbiculatus fruits. Furthermore, DPPH, ABTS, FRAP and reducing power experiments were proceeded to measure the antioxidant activity. The Celastrus orbiculatus fruits were extracted in various times on D.W. so that the antioxidant activity was getting much active as the extracts were stronger. Also, the total polyphenol experiment processed with the 1000 ppm solutions extracted in 1 h, 2 hr, 4 hr, 8 hr, and 12 hr. Each solutions showed the values of 51.88 mg/g, 53.98 mg/g, 62.80 mg/g, 69.94 mg/g and 69.92 mg/g respectively. The highest antioxidant activity showed while the experiments of DPPH, ABTS and FRAP is conveyed with the 8 hour extracted solutions, and the reducing power experiment with the extracted 12 hour solution. WSCs dissolved in D.W. to make them 1000 ppm, the experiments are conducted with each 1000 ppm solutions in the various time zones and volume fraction ratio of the WSCs. Through these experiments, the mixture of the extracted Celastrus orbiculatus fruits and WSCs showed much higher antioxidant activity than only WSCs. As the volume of the Celastrus orbiculatus fruits extraction was getting bigger, the antioxidant activity increased. The result obtained that extracted Celastrus orbiculatus fruits have the antioxidant effect and the effect becomes higher when the WSCs are mixed with the extracted Celastrus orbiculatus fruits.
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KEYWORD
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Celastrus orbiculatus fruits, Antioxidant, Water-soluble chitosan, DPPH, ABTS
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